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[QUOTE=reaper] <section id="matter" property="article" epub:="matter" style="-sizing: border-; clear: both; margin: 1em 0px; color: rgb0, 0, 0; font-family: "Times New Roman"; font-size: 16px; text-align: justify;"><div role="paragraph" -original-font-size="16" -original-line-height="22" style="-sizing: border-; text-indent: 1.4em; line-height: 22px;"><figure id="F1" ="graphic" epub:="figure" style="-sizing: border-; margin: 2em 0px; : relative; text-indent: 0px; font-family: sans-serif; font-size: 0.85em; line-height: 1.2;">[URL=https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/xhtml/f1.xhtml][IMG]https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/graphic/zjm0050880880001.jpeg" height="1253" width="1800[/IMG][/URL]<figcaption style="-sizing: border-; margin: 1em 0px 0.4em;"><span ="ing" -original-font-size="13" -original-line-height="16" style="-sizing: border-; font-weight: 600; font-size: 13px; line-height: 16px;">FIG. 1</span>. Armored L-RNA packaging system. Two expressing vectors were constructed, in which the maturase and the coat protein were expressed from one plasmid [pET-28(b)] and the pac site and the six-target chimeric RNA sequence were produced from the second plasmid (pACYCDuet-1). The pac site was located between SARS3 and HCV. 3V armored L-RNA was produced by inducing and expressing the two-plasmid system.</figcaption></figure><figure id="F2" ="graphic" epub:="figure" style="-sizing: border-; margin: 2em 0px; : relative; text-indent: 0px; font-family: sans-serif; font-size: 0.85em; line-height: 1.2;">[URL=https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/xhtml/f2.xhtml][IMG]https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/graphic/zjm0050880880002.jpeg" height="1800" width="974[/IMG][/URL]<figcaption style="-sizing: border-; margin: 1em 0px 0.4em;"><span ="ing" -original-font-size="13" -original-line-height="16" style="-sizing: border-; font-weight: 600; font-size: 13px; line-height: 16px;">FIG. 2</span>. Characterization of the recombinant RNA packaged in armored L-RNA. Recombinant RNA was isolated from 3V armored L-RNA, fractionated in a denaturing 3% agarose gel, stained with ethidium bromide, and detected by using UV fluorescence. Abbreviations: M, RNA marker; 3V, 3V armored L-RNA recombinant RNA.</figcaption></figure><figure id="F3" ="graphic" epub:="figure" style="-sizing: border-; margin: 2em 0px; : relative; text-indent: 0px; font-family: sans-serif; font-size: 0.85em; line-height: 1.2;">[URL=https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/xhtml/f3.xhtml][IMG]https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/graphic/zjm0050880880003.jpeg" height="1800" width="1615[/IMG][/URL]<figcaption style="-sizing: border-; margin: 1em 0px 0.4em;"><span ="ing" -original-font-size="13" -original-line-height="16" style="-sizing: border-; font-weight: 600; font-size: 13px; line-height: 16px;">FIG. 3</span>. Ethidium bromide-stained 1% agarose gel of RT-PCR amplification products of RNA extracted from 3V armored L-RNA and 3V armored RNA. (A) RT-PCR amplification products of RNA extracted from 3V armored L-RNA. Lane 1, negative control with no template; lanes 2 and 3, negative control without RT; lanes 4 and 5, positive control of pACYC-3V plasmid; lanes 6 to 8, RT-PCR of 3V full-length L-RNA. (B) RT-PCR amplification products of RNA extracted from 3V armored RNA: lane 1, positive control of pET-MS2-3V plasmid using the primers S-SARS1 and HA300RT-A; lane 2, RT-PCR of SARS-CoV1 plus SARS-CoV2 plus SARS-CoV3 plus HCV+HA300 using the primers S-SARS1 and HA300RT-A; lane 3, positive control of pET-MS2-3V plasmid using the primers S-SARS1 and HCV-LAP1; lane 4, RT-PCR of SARS-CoV1 plus SARS-CoV2 plus SARS-CoV3 plus HCV using the primers S-SARS1 and HCV-LAP1; lane 5, positive control of pET-MS2-3V plasmid using the primers S-SARS1 and A-SARS3; lane 6, negative control without RT using primers S-SARS1 and A-SARS3; lane 7, RT-PCR of SARS-CoV1 plus SARS-CoV2 plus SARS-CoV3 using the primers S-SARS1 and A-SARS3; lane 8, RT-PCR of SARS-CoV1 plus SARS-CoV2 using the primers S-SARS1 and A-SARS2; lane 9, positive control of pET-MS2-3V plasmid using the primers S-SARS1 and A-SARS2; lane 10, negative control without reverse transcription using the primers S-SARS1 and A-SARS2.</figcaption></figure><figure id="F4" ="graphic" epub:="figure" style="-sizing: border-; margin: 2em 0px; : relative; text-indent: 0px; font-family: sans-serif; font-size: 0.85em; line-height: 1.2;">[URL=https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/xhtml/f4.xhtml][IMG]https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/graphic/zjm0050880880004.jpeg" height="1004" width="1280[/IMG][/URL]<figcaption style="-sizing: border-; margin: 1em 0px 0.4em;"><span ="ing" -original-font-size="13" -original-line-height="16" style="-sizing: border-; font-weight: 600; font-size: 13px; line-height: 16px;">FIG. 4</span>. Stability study of 3V armored L-RNA. 3V armored L-RNA was added to newborn calf serum to a final concentration of 10,000 and 10,000,000 copies/ml. Samples were incubated at 4°C, 37°C, or room temperature for 0, 1, 2, 4, and 8 weeks. Samples were removed at each time point and were stored at ∼80°C until the completion of the experiment. From these materials, we isolated template RNA for real-time RT-PCR assays. Water was used as a negative control. All RNA templates were assayed in a single run by using an HCV RNA PCR fluorescence quantitative diagnostic kit (Shanghai Kehua Bio-Engineering Co., Ltd.). Real-time RT-PCR was conducted by using LightCycler technology (Roche). The mean for low-copy samples was 67,226 IU/ml (4.83 log<sub style="-sizing: border-; line-height: 1;">10[/SUB]; range, 50,100 to 79,400 IU/ml [range, 4.70 to 4.92 log<sub style="-sizing: border-; line-height: 1;">10[/SUB]]), and the coefficient of variation was 12.9%. The mean for high-copy samples was 29,060,000 IU/ml (7.45 log<sub style="-sizing: border-; line-height: 1;">10[/SUB]; range, 22,900,000 to 41,700,000 IU/ml [range, 7.36 to 7.62 log<sub style="-sizing: border-; line-height: 1;">10[/SUB]]), and the coefficient of variation was 22%.</figcaption></figure><figure id="F5" ="graphic" epub:="figure" style="-sizing: border-; margin: 2em 0px; : relative; text-indent: 0px; font-family: sans-serif; font-size: 0.85em; line-height: 1.2;">[URL=https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/xhtml/f5.xhtml][IMG]https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/graphic/zjm0050880880005.jpeg" height="1280" width="588[/IMG][/URL]<figcaption style="-sizing: border-; margin: 1em 0px 0.4em;"><span ="ing" -original-font-size="13" -original-line-height="16" style="-sizing: border-; font-weight: 600; font-size: 13px; line-height: 16px;">FIG. 5</span>. Calibration of the real-time RT-PCR assay for HCV, SARS-CoV2, and HA300 (H5N1). First, the quantified 3V armored L-RNA was diluted with newborn calf serum 10-fold serially to obtain 100, 1,000, 10,000, 100,000, and 1,000,000 copies/ml. We used the National Reference material for HCV RNA (GBW09151, 2.26 × 10<sup style="-sizing: border-; line-height: 1;">3[/SUP] IU ml<sup style="-sizing: border-; line-height: 1;">−1[/SUP] to 4.22 × 10<sup style="-sizing: border-; line-height: 1;">7[/SUP] IU ml<sup style="-sizing: border-; line-height: 1;">−1[/SUP]) to calibrate the serial dilutions of chimeric armored L-RNA and then used the calibrated L-RNA to prepare calibrators for the two real-time RT-PCR assays. From these materials, we isolated RNA template for RT-PCR assays. Newborn calf serum was used as a negative control. Real-time RT-PCR was conducted on a LightCycler thermal cycler (Roche). (A) Log concentration of the international standard for HCV RNA versus the cycle number for the HCV RT-PCR; (B) amplification curve for the HCV RT-PCR assay; (C) amplification curve for the SARS-CoV2 RT-PCR assay; (D) amplification curve for the HA300 (H5N1) RT-PCR assay.</figcaption></figure><figure id="T1" ="table" epub:="figure" style="-sizing: border-; margin: 2em 0px; : relative; text-indent: 0px; font-family: sans-serif; font-size: 0.85em; line-height: 1.2;"><figcaption style="-sizing: border-; margin: 1em 0px 0.4em;"><span ="ing" -original-font-size="13" -original-line-height="16" style="-sizing: border-; font-weight: 600; font-size: 13px; line-height: 16px;">TABLE 1</span>. Primers used for PCR amplification <span ="nonlinear-" -original-font-size="13" -original-line-height="16" style="-sizing: border-; font-size: 13px; line-height: 16px;">([URL=https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/xhtml/t1.xhtml]Table view[/URL])</span></figcaption><div ="table-wrap" -original-font-size="13" -original-line-height="16" style="-sizing: border-; overflow: auto; padding-bottom: 0.75em; text-align: initial; font-size: 13px; line-height: 16px; break-inside: auto;"><table style="border-bottom: 2px solid rgb136, 136, 136; border-collapse: collapse; border-spacing: 0px; border-top: 2px solid rgb136, 136, 136; line-height: 1.2; min-width: 100%;"><t style="-sizing: border-; border-bottom: 1px solid rgb136, 136, 136;"><tr --align="center" --valign="bottom" style="-sizing: border-; text-align: center; vertical-align: bottom;"><th style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; font-weight: 400;">Primer name</th><th style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; font-weight: 400;">Primer sequence (5′ to 3′)[URL=https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/xhtml/index.xhtml#T1FN1]<sup style="-sizing: border-; line-height: 1;]<i style="-sizing: border-;]a[/I][/SUP][/URL]</th></tr></t><t style="-sizing: border-;"><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">S-MC</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">CG<span -style="underline" -original-font-size="13" -original-line-height="16" style="-sizing: border-; text-decoration-line: underline; line-height: 16px;">GGATCC</span>TGGCTATCGCTGTAGGTAGCC</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">A-MC</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">CCC<span -style="underline" -original-font-size="13" -original-line-height="16" style="-sizing: border-; text-decoration-line: underline; line-height: 16px;">AAGCTT</span>ATGGCCGGCGTCTATTAGTAG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">S-SARS1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">TATCCAAAATGTGACAGAGCCATG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">LAP-SARS1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">ACGCTGAGGTGTGTAGGTGCAGGTAAGCGTAAAACTCATCCAC</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">A-SARS2</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">TAACCAGTCGGTACAGCTACTAAG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">LAP-SARS2</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">AGTTTTACGCTTACCTGCACCTACACACCTCAGCGTTGATATAAAG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">A-SARS3</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">ACTACGTGATGAGGAGCGAGAAGAG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">LAP-SARS3</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">AGCTGTACCGACTGGTTAACAAATTAAAATGTCTGATAATGGACCCC</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">LAP-SARS2<sup style="-sizing: border-; line-height: 1;">+[/SUP]</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">ATCAGACATTTTAATTTGTTAACCAGTCGGTACAGCTACTAAG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">S-HCV</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;"><span style="-sizing: border-; font-weight: 600;">ACATGAGGATCACCCATGT</span>GGCGACACTCCACCATAGATCACTC</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">HCV-LAP1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">ATGTAAGACCATTCCGGCTCGCAAGCACCCTATCAGGCAGTAC</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">A-HA300</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">GAATCCGTCTTCCATCTTTCCCCCACAGTACCAAAAGATCTTC</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">HA300LAP</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">CTGATAGGGTGCTTGCGAGCCGGAATGGTCTTACATAGTGGAG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">M+SLAP1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">ATGGCTCTGTCACATTTTGGATAGAGTAGCTGAGTGCGACCTCCTTAG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">M+SLAP2</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">AAGGAGGTCGCACTCAGCTACTCTATCCAAAATGTGACAGAGCCATG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">FIVELAP1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">CATGGGTGATCCTCATGTACTACGTGATGAGGAGCGAGAAGAG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">FIVELAP2</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">CGCTCCTCATCACGTAGTACATGAGGATCACCCATGTGGC</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">OverlapA′</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">CC<span -style="underline" -original-font-size="13" -original-line-height="16" style="-sizing: border-; text-decoration-line: underline; line-height: 16px;">TTAATTAA</span> CCCACAGTACCAAAAGATCTTCTTG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">M300-S′</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">TT<span -style="underline" -original-font-size="13" -original-line-height="16" style="-sizing: border-; text-decoration-line: underline; line-height: 16px;">GGCCGGCC</span> GAGTCTTCTAACCGAGGTCGAAACG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">overlap-A</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">CCCACAGTACCAAAAGATCTTCTTG</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">M300RT-S</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">GGATTTGTATTCACGCTCACC</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">HA300RT-A</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">TGGGGATGATCTGAATTTTCTC</td></tr></t></table>[/DIV]<div ="notes" -original-font-size="13" -original-line-height="16" style="-sizing: border-; margin: 1em 0px 0px; overflow: auto; font-size: 13px; line-height: 16px;"><div ="labeled" role="doc-footnote" epub:="footnote" -original-font-size="13" -original-line-height="16" style="-sizing: border-; line-height: 16px;"><div ="label" -original-font-size="13" -original-line-height="16" style="-sizing: border-; display: table-cell; padding-right: 0.5em; line-height: 16px;"><i style="-sizing: border-;">a[/I][/DIV]<div id="T1FN1" role="paragraph" -original-font-size="13" -original-line-height="16" style="-sizing: border-; display: table-cell; line-height: 16px;">BamHI, HindIII, FseI, and PacI restriction sites are indicated by underscoring; a C vairiant is indicated in boldface type.[/DIV][/DIV][/DIV]</figure><figure id="T2" ="table" epub:="figure" style="-sizing: border-; margin: 2em 0px; : relative; text-indent: 0px; font-family: sans-serif; font-size: 0.85em; line-height: 1.2;"><figcaption style="-sizing: border-; margin: 1em 0px 0.4em;"><span ="ing" -original-font-size="13" -original-line-height="16" style="-sizing: border-; font-weight: 600; font-size: 13px; line-height: 16px;">TABLE 2</span>. Thermal cycle conditions for the three different kits used in real-time RT-PCR assays <span ="nonlinear-" -original-font-size="13" -original-line-height="16" style="-sizing: border-; font-size: 13px; line-height: 16px;">([URL=https://journals.asm.org/reader/content/177da1f9043/10.1128/JCM.02248-07/format/epub/EPUB/xhtml/t2.xhtml]Table view[/URL])</span></figcaption><div ="table-wrap" -original-font-size="13" -original-line-height="16" style="-sizing: border-; overflow: auto; padding-bottom: 0.75em; text-align: initial; font-size: 13px; line-height: 16px; break-inside: auto;"><table style="border-bottom: 2px solid rgb136, 136, 136; border-collapse: collapse; border-spacing: 0px; border-top: 2px solid rgb136, 136, 136; line-height: 1.2; min-width: 100%;"><t style="-sizing: border-; border-bottom: 1px solid rgb136, 136, 136;"><tr --align="center" --valign="bottom" style="-sizing: border-; text-align: center; vertical-align: bottom;"><th style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; font-weight: 400;">Program</th><th style="-sizing: border-; padding: 0.3em 0.4em 0px; font-weight: 400;">No. of cycles</th><th style="-sizing: border-; padding: 0.3em 0.4em 0px; font-weight: 400;">Temp (°C)</th><th style="-sizing: border-; padding: 0.3em 0.4em 0px; font-weight: 400;">Incubation time (min:s)</th><th style="-sizing: border-; padding: 0.3em 0.4em 0px; font-weight: 400;">Temp transition rate (°C/s)</th><th style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; font-weight: 400;">Acquisition mode</th></tr></t><t style="-sizing: border-;"><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">HCV</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;"> </td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">50</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">25:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 2</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">94</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">2:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 3</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">5</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">93</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">3:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">55</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">15:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">2</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">72</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">15:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 4</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">42</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">93</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">3:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">60</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">45:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">Single</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 5</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">40</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">30:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">H5N1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;"> </td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">42</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">30:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 2</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">92</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">3:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 3</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">5</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">92</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">10:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">45</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">30:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">72</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">1:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 4</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">40</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">92</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">10:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">60</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">30:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">Single</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 5</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">40</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">0:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="left" --valign="top" style="-sizing: border-; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;">SARS-Cov2</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;"> </td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">42</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">30:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 2</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">92</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">3:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 3</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">5</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">92</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">10:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">52</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">2</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">72</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">30:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">40</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">92</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">5:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 4</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;"> </td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">60</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">30:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">Single</td></tr><tr --align="center" --valign="top" style="-sizing: border-; text-align: center; vertical-align: top;"><td --align="left" -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px 0px; vertical-align: top; text-align: left; font-size: 13px; line-height: 16px;"> 5</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">1</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">40</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">10:00</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0.4em 0px; vertical-align: top; font-size: 13px; line-height: 16px;">20</td><td -original-font-size="13" -original-line-height="16" style="-sizing: border-; padding: 0.3em 0px 0px 0.4em; vertical-align: top; font-size: 13px; line-height: 16px;">None</td></tr></t></table>[/DIV]</figure>[/DIV]</section><section id="backmatter" property="sa:articleBackMatter" epub:="backmatter" style="-sizing: border-; clear: both; margin: 1em 0px; font-size: 0.9em; color: rgb0, 0, 0; font-family: "Times New Roman"; text-align: justify;"><section id="acknowledgments" role="doc-acknowledgments" epub:="acknowledgments" style="-sizing: border-; clear: both; margin: 1em 0px;"><h2 -original-font-size="17" -original-line-height="24" style="-sizing: border-; font-weight: 300; text-align: left; margin: 0px; font-size: 17px; line-height: 24px;">Acknowledgments</h2><div role="paragraph" -original-font-size="14" -original-line-height="20" style="-sizing: border-; text-indent: 1.4em; font-size: 14px; line-height: 20px;">This study was supported in part by the SEPSDA project of the European Commission (under no. Sp22-CT-2004-003831), the National Natural Science Foundation of China (30371365 and 30571776), and the Capital Medicine Development Foundation of Beijing (2002-3041).[/DIV]</section><section id="bibliography" role="doc-bibliography" epub:="bibliography" style="-sizing: border-; clear: both; margin: 1em 0px;"><h2 -original-font-size="17" -original-line-height="24" style="-sizing: border-; font-weight: 300; text-align: left; margin: 0px; font-size: 17px; line-height: 24px;">REFERENCES</h2><div ="labeled" role="doc-biblioentry" epub:="biblioentry" -original-font-size="14" -original-line-height="20" style="-sizing: border-; font-size: 14px; line-height: 20px;"><div ="label" -original-font-size="14" -original-line-height="20" style="-sizing: border-; display: table-cell; padding-right: 0.5em; text-align: right; line-height: 20px;">1.[/DIV]<div id="R1" ="citations" -original-font-size="14" -original-line-height="20" style="-sizing: border-; display: table-cell; padding: 0px 0px 0.4em; line-height: 20px;"><span ="citation" -original-font-size="14" -original-line-height="20" style="-sizing: border-; line-height: 20px;"><b style="-sizing: border-;">Argetsinger, J., and G. 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